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SIMPLE PCR TECHNIQUE FOR DETECTING AND DIFFERENTIATING BACTERIAL PATHOGENS
A simple polymerase chain reaction procedure is described for the detection and differentiation of Shigella from other pathogenic Escherichia coli isolates, such as EIEC and EPEC. Serotype specific primers derived from the rfc genes of different Shigella strains are used to identify the most prominents Shigella serotypes, such as S. sonnei, S. flexneria 1 through 5, and S dysenteriae 1. More than 95% of Shigellosis cases reported could be identified by the serotype specific primers described.
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